FLOW INJECTION ANALYSIS AND SPECTROPHOTOMETRIC DETERMINATION OF NIFEDIPINEIN PHARMACEUTICAL FORMULATION

FLOW INJECTION ANALYSIS AND SPECTROPHOTOMETRIC DETERMINATION OF NIFEDIPINEIN PHARMACEUTICAL FORMULATION

  • انتصار مظفر السعيدي
  • سديم صبحي عبد
Keywords: Key words: Nifedipine, Pyrocatechol, Oxidative coupling reaction, Spectrophotometry, Flow injection analysis

Abstract

A new simple and sensitive spectrophotometric method is described for quantification of Nifedipine (NIF) and their pharmaceutical formulation. The selective method was performed by the reduction of NIF nitro group to yield primary amino group using zinc powder with hydrochloric acid. The produced aromatic amine was submitted to oxidative coupling reaction with pyrocatechol and ammonium ceric nitrate to form orange color product measured spectrophotometrically with maximum absorption at 467nm. The product was determined through flow injection analysis (FIA) system and all the chemical and physical parameters were optimized. The concentration range from 5.0 to 140.0 μg.mL-1 was obeyed Beer’s law with a limit of detection and quantitation 1.48 and 4.96 μg.mL-1 respectively. Agoodprecision,low scattering point of the calibration graph and good accuracyin addition, FIA introduced a good linear range with acceptable sensitivity. High correlation coefficient (0.9996) was found. The proposed method was successfully applied to assay NIF and its pharmaceutical dosage also could be utilized for pharmaceutical routine analysis of the drug.

Author Biographies

انتصار مظفر السعيدي

1Department of chemistry, College of Science, University of Baghdad, Baghdad, Iraq

سديم صبحي عبد

2Asst. Prof. Ph.D. Department of chemistry, College of science, University of Baghdad, Baghdad, Iraq

Published
2019-04-25