EFFECT OF ADDING QUERCETIN TO TRIS EXTENDER IN SOME SEMEN CHARACTERISTICS OF AWASSI RAMS AFTER DIFFERENT PERIODS OF CRYOPRESERVATION

Abstract

ABSTRACT

The study evaluated the effect of adding quercetin to some characteristics of the sperm of the ram. This study was conducted in the animal field, Department of Animal Production, College of Agricultural Engineering Science, University of Baghdad for the period 5/12/2021 to 20/2/2022. In this experiment, 3 rams were used at the age of 2-2.5 years and weighed 50-55 kg. The semen was collected early in the morning and once a week and the semen was pooled to remove the individual differences. The treatments were divided: quercetin-free control group, treatment T1 (3 µL/mL quercetin), T2 treatment (6 µL/mL quercetin), T3 treatment (9 µL/mL quercetin). The result of the study showed a significant increased (p<0.01) of T2 treatment in the percentage of individual sperm motility during the cooling period (2 and 24 h). T3 treatment showed a significant increase (p<0.05) at (48 and 72 h) of individual motility. On the other hand, T2 treatment showed a significant increased (p<0.01) in the viability of sperm at (2 and 24 h) of cooling preservation, while T3 treatment showed a significant increase at (48 and 72 h) cooling preservation for the viability of sperm. The result indicated that adding quercetin does not effect of total abnormality of sperm for all treatments. The result presented  significant increased (p<0.05) percentage of  HOST for T2 and T3 treatments. The results showed a significant increase at ( p<0.01) for T2 treatment at (2 and 48 h) on the other hand T1 showed a significant increase at 72 h. for the same character.