ASSOCIATION OF THE C2077G SNP IN GDF-9 GENE WITH THE PRODUCTIVE PERFORMANCE OF LOCAL AND SHAMI GOAT

Abstract

The study was conducted on a sample of 44 local goats and 52 Sham goat at the Ruminant Research Station of the Agricultural Research Department / Ministry of Agriculture. Nucleotide sequencing technology was used to detect the SNP (C2077G) in the studied segment of the GDF-9 gene, in order to determine the relationship of the genotypes resulting from the mentioned mutation to the growth traits, milk yield  and fertility. The genetic polymorphism are wild (CC), Heterozygous (CG), and mutant (GG). The percentage of individuals carrying the wild genotype was superior to each of the individuals carrying the mutant and hybrid genotype (52.27, 45.45, 2.27)%, respectively, in the local sample, while the percentage of individuals was superior The carriers of the wild genotype over each of the individuals carrying the heterozygous and mutant genotype were (65.38, 32.69 and 1.92)%, respectively, in the Shami goat sample, and the allelic frequency of the wild allele C in both Goat was (0.53, 0.82%), compared to the mutant allele G (0.47, 0.18). The results showed that there was a significant effect of the polymorphism of the mutation (C2077G) in the characteristics of the weaning weight and the current weight in the local goat and in favor of individuals with hybrid genotype (CG) (20.70, 38.21) kg, respectively, but in the sample of the Shami goats, an effect was found significant of the genetic variation caused by the mutation (C2077G) in birth weight and weaning weight in favor of individuals carrying the hybrid genotype (3.04, 22.20) kg, respectively, The results showed a significant effect of the polymorphism of the C2077G SNP on litter size in local and Shami goats in favor of individuals carrying the hybrid genotype CG (1.49, 1.76) births per litter. The mutation (C2077G) in the GDF-9 gene was recently discovered and is not registered in NCBI, so it has no identification number( rs ) .