Study of Antioxidation Activity of Sulfated Polysaccharide Extracted From Green Algae Enteromorpha sp.

Study of Antioxidation Activity of Sulfated Polysaccharide Extracted From Green Algae Enteromorpha sp.

  • Amal Kadhim Ghadban
  • Khalid Hassak Abdulhasan
  • Mazin J. Hindi
Keywords: Enteromorpha, Sulfated Polysaccharides, Antioxidation Activity.

Abstract

Samples of the green algae were collected from water of Shatt al-Arab in Garmat Ali in Basra. After purification, the green algae identified on Enteromorpha sp. The samples were dried and milled, then sulfated polysaccharides were extracted with hot water at 90°C precipitated with absolute ethanol, dialysed and lyophilized. The chemical composition was total sugars 56.4%, protein 1.3% and sulfur 19.7%. Antioxidation activity of sulfated polysaccharides was studied by four method and included estimation of ability of scavenging hydroxylated radicals, the results showed an increased in ability with increasing concentrations. Ability of scavenging and was 59.86% at the concentration of 2.5 mg/ ml, but BHT was 81.36%. Ability of scavenging of hydrogen peroxide at was 59.61% at 1000 µg/ ml, but gallic acid was 82.67% at the same concentration. Ability of chelating ferrous ion was 51.19% at 5 mg/ ml, but EDTA was 98.72%.Reductive capacity was also measured, the maximum absorbance was 1.436 nm for 1000 µg/ mL, but ascorbic acid was 1.736 nm.

Author Biographies

Amal Kadhim Ghadban

Department of Food Science
College of Agriculture
University of Basra

Khalid Hassak Abdulhasan

Department of Food Science
College of Agriculture
University of Baghdad

Mazin J. Hindi

Department of Food Science
College of Agriculture
University of Baghdad

Published
2015-06-29