PREPARATION OF PROTEIN CONCENTRATES FROM THE WASTE OF CARP FISH (Cyprinus carpio) AS AN ALTERNATIVE TO THE NITROGEN SOURCE FOR THE GROWTH OF LACTIC ACID BACTERIA

Abstract

This study aimed to prepare protein concentrates from carp fish (Cyprinus carpio) waste as an alternative to nitrogen sources for the growth of lactic acid bacteria. Two types of protein concentrates were prepared using the pH shift method (Acidic protein concentrate at pH 2/4.5 and Basic protein concentrate at pH 12/4.5). The results showed that the process of removing the fat and precipitating proteins at the isoelectric point contributed to increasing protein recovery in the protein concentrates, the number of Lactobacillus acidophilus, Lactobacillus rhamnosus GG, and Bifidobacterium animalis colonies in the alternative media was lower than the commercial DeMan, Rogosa and Sharpe (MRS) medium, the number of Lactobacillus plantarum colonies was approximated to the commercial media, while the number of Bifidobacterium infantis colonies was higher than the commercial media.