STUDYING THE EFFECT OF PUMPKIN PEEL NANO EXTRACT ON INHIBITING THE ACTIVITY OF SOME MICROORGANISMS

Abstract

The study aimed to evaluate the anti-alcoholic activity of pumpkin and nanoscale extract against some types of bacteria. The results showed that the Cu/Cs-NPs was more effective than the methanolic extract of pumpkin peel on all types of Used becteria in the study, despite the use of the plant extract at higher concentrations. Concentrations, the were 100 and 200 mg/ml, while the nano composite was used in lower concentrations, which were 16 and 32 mg/ml. The Cu/Cs-NPs gave the highest inhibition zones of (20.33, 17.33, 12.67, 16.33) mm at a concentration of 32 mg/ml for each. Of the For each bacteria Staphylococcus aureus, Salmonella Typhi, Pseudomonas aeruginosa, and Escherichia coli , respectively, while the methanolic extract of Pumpkin peels gave the highest zones of inhibition of (19.67, 16.67, 10.33, 67.15) mm at a concentration of 200 mg/ml for each of the bacteria. Staphylococcus aureus, Salmonella Typhi, Pseudomonas aeruginosa, and Escherichia coli, respectively. The minimum inhibitory concentration (MIC) values for the Cu/Cs-NPs on all isolates of Escherichia coli and Pseudomonas aeruginosa bacteria were 0.5 and 1 mg/ml, respectively, and 2 mg/ml,. On all isolates of Salmonella Typhi bacteria, 0.5 mg/ml for strains No. 1 and 4, and 0.25 mg/ml on isolates 2 and 3 for Staphylococcus aureus bacteria, while the MIC values for the methanolic extract of Pumpkin peels reached 8 mg/ml on all isolates of Escherichia coli bacteria, with the exception of Isolate No. 2 had a minimum inhibitory concentration (MIC) of 16 mg/ml and 64 mg/ml on all isolates of Pseudomonas aeruginosa bacteria, and the MIC values of the methanolic extract of Pumpkin peels reached 8 and 4 mg/ml on all isolates of Salmonella bacteria. Enterococcus and Staphylococcus aureus, respectively. The nanocomposite also showed high inhibitory activity compared to the methanolic extract against the tested fungi.