ISOLATION AND IDENTIFICATION OF PSEUDOMONAS SP THAT PRODUCE LIPASE

ISOLATION AND IDENTIFICATION OF PSEUDOMONAS SP THAT PRODUCE LIPASE

  • مصطفى إبراهيم خليفة
  • جاسم محمد عودة
Keywords: Isolation, diagnosis, 16S rRNA gene, Lipase.

Abstract

15 local isolates of Pseudomonas were obtained from 35 samples from several sources such as soil, water and some high-fat foods. The ability of isolates to produce lipase was measured by the size of the clarification zone formed around the colonies on the lipase production medium and by measuring the enzymatic activity and specific enzymatic activity, the isolate M3 was found to be the most efficient for production of the enzyme, This isolate was identified by microscopic, morphological, some biochemical tests and genetic diagnosis of 16S gene sequences by using the (PCR) technique, and then comparing the results obtained with the National Center for Biotechnology Information (NCBI) website, M3 isolates were found to be Pseudomonas aeruginosa .

Author Biographies

مصطفى إبراهيم خليفة

1Department of Food Sciences, College of Agriculture, University of Baghdad, Bagdad, Iraq

جاسم محمد عودة

2Department of Food Sciences, College of Agriculture, University of Baghdad, Bagdad, Iraq

Published
2018-08-12