ISOLATION AND IDENTIFICATION OF BACTERIA THAT PRODUCE AMYLASE

ISOLATION AND IDENTIFICATION OF BACTERIA THAT PRODUCE AMYLASE

  • Jasim M Awda
  • Ali H. Fayyadh
Keywords: Isolation, Diagnosis, 16S rRNA, Amylase.

Abstract

Thirty six bacteria were isolated from various sourcesc (soil, starch, cooked rice and other foods) and subjected to a series of primary screening tests to obtain the optimal isolation to production of amylase. The volume of producing zone by logal indicator for (Seven) isolates of the secondary screening by measuring the enzymatic activity and specific enzymatic activity. The isolate A4 was found to be the most efficient for production of amylase. Then this isolate was diagnosed through microscopic, vitek 2 system technique. in addition by gentic diagnesis through gene 16s of the genes nitrogen bases by use the polymerase chain reaction (PCR) which reached 1256 bases. In comparison to the available information at the National Center for Biotechnology Information (NCBI), the isolation was found to be Bacillus subtilis

Author Biographies

Jasim M Awda


1. Assis. Prof. Dr. Department of Food Sciences, College of Agriculture, University of Baghdad, Bagdad, Iraq. Dr. [email protected] coagri. uobaghdad.edu.iq

Ali H. Fayyadh

Department of Food Sciences, College of Agriculture, University of Baghdad, Bagdad, Iraq

Published
2022-11-20